The Hidden Leaf Village itself could become the main antagonist. Since Naruto turns evil, the village that once nurtured him would now be against him. The village elders and some of the ninja who oppose Naruto's new evil ways would be in conflict with him. For example, Kakashi might be forced to fight against Naruto to protect the village.
Sasuke could also be an antagonist. If Naruto turns evil, Sasuke, who has his own sense of justice, would likely oppose Naruto. Their relationship would be completely reversed. Sasuke might see Naruto as a threat to the world he wants to create or protect, and they could engage in epic battles.
The Hidden Leaf Village elders could be main antagonists. If Naruto turns dark because he feels betrayed by the village, they would be the ones representing the establishment he is against. Their conservative and sometimes self - serving ways could be the catalyst for Naruto's transformation, and then they would be the ones trying to stop him as he becomes a threat to their power and the village's traditional order.
The Hidden Leaf Village elders could be antagonists. If Naruto turns dark, they might see him as a threat to their power and try to stop him. Their actions against Naruto could further fuel his descent into darkness.
The Akatsuki could also be the main antagonists. Since they are after the bijuu, they would surely target Naruto once he has this special relationship with them. They might try more elaborate schemes to capture the bijuu from Naruto and his 'harem' of sorts.
Maybe there is an ancient wolf demon spirit that is corrupted and sees Naruto as a threat to its reign. This spirit would try to either kill Naruto or corrupt him further, causing chaos in the world.
The main antagonists could be the ones who banished Naruto in the first place. Maybe it was a group of elders in the village who feared Naruto's power and wanted to get rid of him. They would see Naruto as a threat now that he is becoming the Onikage and would try to stop him from returning to the village or causing any changes.
It could be Orochimaru. He's always interested in strange and powerful forms, and Naruto's new wolf - bijuu form would be something he'd want to study or even control for his own evil purposes.
In this fanfiction, the main antagonists could be a secret organization that has been studying the Six Paths powers in the shadows. They see Naruto as a threat to their plans of world domination. They could have their own powerful members with unique abilities that are specifically designed to counter Naruto's newfound powers as the Sage of the Six Paths. They might try to manipulate events and turn Naruto's friends against him to weaken his position.
Another possibility is that a new, unknown group from outside the village emerges as the antagonists. They see Naruto as a threat to their own plans for domination or chaos, and they target the village under his leadership. This group could have unique powers and abilities that Naruto and the villagers have never faced before, making it a real test for Naruto as the Hokage.
The main antagonist could be the evil Naruto himself. Since he has turned to the dark side, his actions and motives would be against the well - being of the village and those around him, including Kushina. He might be the source of conflict and chaos.
Anko might not be a traditional antagonist, but if she is siding with Evil Naruto for some reason, she could be seen as an antagonist from the perspective of the other villagers. However, it's also possible that she is being manipulated herself, in which case the true antagonists would be those doing the manipulating.