It could be HIM. Since he has always been a major villain in the Powerpuff Girls universe, he might find a new way to turn the girls evil in this fanfiction. Maybe he uses a more insidious form of his magic this time.
In this fanfiction, it might be that a powerful dark magic has seeped into Townsville. The Powerpuff Girls, being so in - tune with the city, are the first to be affected. The magic corrupts their sense of right and wrong. They then use their powers for self - gain and to dominate the city rather than protect it. Their once - cute and friendly appearance may also take on a more menacing look as they turn evil.
A powerful music maestro who is jealous of Allegro's natural talent. He wants to steal Allegro's musical essence to make himself the greatest musician in the world and is willing to use any means, including attacking the Powerpuff Girls.
Mojo Jojo would be a likely main antagonist. He's always trying to outwit the Powerpuff Girls, and using hypnosis would be just another devious plan in his arsenal.
It could be a powerful witch. This witch is jealous of the Powerpuff Girls' beauty and power. So she casts a spell that mutes their beauty, making them look ordinary. This not only affects their appearance but also weakens their powers. The witch wants to take over Townsville while the girls are in this weakened state.
The Spirit of the Millennium Items could also be the main antagonist. They were initially in harmony with Atem, but with his turn to evil, they might try to resist his control. Since they are powerful entities in their own right, they could become a force against Atem. They would not want to be used for evil purposes and would fight back against Atem's newfound malevolence.
Voldemort could still be the main antagonist. Even though Harry has turned into a girl, Voldemort's goal of getting rid of Harry would remain the same. He might even see Harry's new form as an opportunity to weaken him, thinking that the change would make Harry less of a threat magically or in terms of leadership.
Perhaps a new character could be the main antagonist in this fanfiction. A power - hungry alchemist who discovers the Turtles' mutation and wants to use their baby forms to create an army of mutant slaves. This alchemist would stop at nothing to get his hands on the baby Turtles and experiment on them.
Gary could be the main antagonist. He has always been Ash's rival and seeing Ash turn evil, he might feel responsible to stop him. Gary could gather his own team of powerful Pokémon and use his wits to oppose Ash at every turn.
It could be the Green Goblin. He might manipulate Spider Man in some way, perhaps by tricking him into believing that the only way to protect the city is to rule it with an iron fist. This would slowly corrupt Spider Man's good intentions.
Dumbledore could be the main antagonist. Harry might see Dumbledore as having used him all along for some grand plan. If Harry turns evil, he would want to break free from Dumbledore's influence. Dumbledore, with his power and wisdom, would then try to stop Harry from causing too much harm, thus becoming the main opposing force.