The Khaos Brigade could be a major antagonist. Their goal of causing chaos would put them at odds with Gohan's sense of justice.
It could be an evil dragon from the DxD lore that decides Gohan is a threat to its plans. Since Gohan is so powerful, the dragon might see him as the only obstacle in its path to rule the DxD world.
If it's a crossover with the One Piece world, Blackbeard could be a potential bad guy. His ability to steal Devil Fruit powers and his ambition to become Pirate King could lead to a clash with Gohan. Gohan's pure - hearted nature would be in opposition to Blackbeard's more devious ways, and their battle could be epic, with Gohan's ki powers against Blackbeard's dark powers.
Another possibility is that the main antagonists could be the leaders of the other ninja villages. They might view Naruto's godlike and rogue status as a danger to the balance of power among the villages. So, they could form an alliance against Naruto, leading to a lot of political intrigue and conflict in the story.
Perhaps there are dark forces from another dimension. These forces might be attracted to Naruto's power and want to either steal it or destroy him to prevent him from using it against them. They could be powerful demons or sorcerers with their own motives.
Well, it could be a powerful gamer who has a grudge against Gohan. Maybe this gamer has lost to Gohan in some important in - game tournaments and now wants revenge. So he devises all kinds of devious plans to undermine Gohan's progress in the game.
It could be an old enemy of the group who has a personal vendetta against Issei. This antagonist might be jealous of Issei's power or popularity within the group and plots to turn everyone against him. They could spread false rumors or create false evidence to make it seem like Issei has betrayed the group.
The devils who oppose Naruto's presence and his new - found influence in the DxD world could be main antagonists. They might see him as a threat to their power structure and the way things are done in their society.
The devils who oppose Gilgamesh's presence in the Highschool DxD world could be main antagonists. They might see him as a threat to their power and influence in the school.
In this kind of fanfiction, the Fallen Angels could be main antagonists. They might see Naruto and Sasuke as threats to their plans in the DxD world.
Well, in this fanfiction, the Akatsuki could be the main antagonists. They would view Naruto's godlike powers as a major threat to their goals. Since Naruto has been trained by Shendu, his abilities would be unpredictable and extremely powerful. They might band together to try and take Naruto down. Additionally, there could be some ancient demons or spirits that are awakened by Naruto's new powers and they see him as an intruder in their realm, so they become antagonists as well. They would use their own dark magic and powers to oppose Naruto.
The village elders could be the main antagonists. They might see Naruto's godlike powers as a threat to their traditional way of ruling the village and decide to get rid of him. Another option could be a secret organization within the village that wants to use Naruto's power for their own evil purposes and when he refuses, they turn on him.