Perhaps a powerful and rogue Pokémon trainer who has a vendetta against Ash and wants to stop him from achieving his goals in the regions. This trainer might have some extremely strong Pokémon that are difficult to defeat.
It could be a new evil team that wants to control all the Pokémon for their own greedy purposes.
There might be an ancient evil force that has been awakened in the regions. It could be a spirit or a powerful entity that tries to disrupt the balance of the Pokémon world, and Ash has to face it to save the regions he loves.
Pikachu, of course. It's always been Ash's closest partner.
Maybe Ash will encounter new and rare Pokémon in the regions this time. He could also meet some old friends and have epic battles together.
Perhaps Ash is on a quest to complete a special Pokémon research project this time. He travels through the regions, interviewing different trainers and Pokémon experts. He might get involved in some local Pokémon traditions and competitions that he hadn't experienced before. His Pikachu will surely be by his side, and they might develop new battle strategies against the diverse Pokémon they encounter.
A group of rival trainers could be the antagonists. These trainers are jealous of Ash's skills and Erika's knowledge. They constantly try to sabotage their journey, for example, by setting traps or spreading false rumors about dangerous Pokémon in the areas they are about to travel through.
He might meet some of his old friends again, like Brock or Misty. Maybe they will join him on his new journey through the regions.
In this fanfiction, Ash might encounter new Pokémon species in the regions he revisits. He could also form new friendships or rivalries with trainers he meets along the way.
Perhaps a powerful and mysterious trainer. This trainer is jealous of Ash's connection with his Pokemon and his ability to conduct these experiments. He wants to prove that he is the better trainer. So he tries to disrupt Ash's experiments by challenging him to battles at inconvenient times. He also spreads false rumors about Ash's experiments being dangerous to other Pokemon, which causes some local Pokemon communities to be hostile towards Ash.
In some cases, it could be other time - traveling vampires or supernatural beings who have their own agendas. They might not want Bella to interfere with the past as it could disrupt their own plans or power bases. These antagonists could be more shadowy figures, working behind the scenes to thwart Bella's efforts.
A group of werewolves who are against Klaus's rule could also be the antagonists. They might view the new baby as another potential threat from Klaus and Hayley's union. So they plan to disrupt the pregnancy to prevent the birth of a half - vampire, half - werewolf child that could potentially be very powerful.
Team Rocket could still be antagonists. Even if Ash goes back in time, they might be up to their old tricks of trying to steal Pokémon. Only this time, Ash has the advantage of knowing their future plans and can thwart them more easily.