What chapter should I bookmark for the first truly irreversible loss tied to Manus?

Chapter 349, ", Killed, Bring the Head Back", is the clearest early answer because the violence around Manus becomes final instead of hypothetical. The chapter gives readers a real answer by opening on He had originally planned to haul away several train cars, but now... and ending on Put him down, take his head, and bring it back.. In story terms, the danger stops being abstract and the conflict around faction conflict becomes immediate, forcing the surrounding cast to react under pressure.

Chapter 237, "The Summons' Massacre", is the clearest early answer because the violence around Cerbe becomes final instead of hypothetical. The chapter gives readers a real answer by opening on The air was thick with the stench of blood and fear. and ending on If we warn the others, we can—" A rustling sound interrupted him..

Chapter 109, "Minor Flame Magic Skill", is the clearest early answer because the violence around Damien becomes final instead of hypothetical. The chapter gives readers a real answer by opening on Damien wandered through the forest, his steps slow and unhurried. and ending on They drained life from their surroundings to sustain their unnatural....

Chapter 522, "Massacre In The East Base", is the clearest early answer because the violence around Damien becomes final instead of hypothetical. The chapter gives readers a real answer by opening on There was no organized ranks nor was there a marching formation. and ending on A presence had been added to the mix..

Chapter 13, "Killing the Chicken to Scare the Monkey", is the clearest early answer because the violence around Chicken becomes final instead of hypothetical. The chapter gives readers a real answer by opening on Sensing that something was off with Jiang Yan, Jiang Yan dismissed...

Chapter 13, "Killing the Chicken to Scare the Monkey", is the clearest early answer because the violence around Chicken becomes final instead of hypothetical. The chapter gives readers a real answer by opening on Sensing that something was off with Jiang Yan, Jiang Yan dismissed...

Chapter 826, "Deadlock", is the clearest early answer because the violence around Yu becomes final instead of hypothetical. The chapter gives readers a real answer by opening on The Li Family Master had spoken, so Father Yu quickly followed suit...

Chapter 826, "Deadlock", is the clearest early answer because the violence around Yu becomes final instead of hypothetical. The chapter gives readers a real answer by opening on The Li Family Master had spoken, so Father Yu quickly followed suit...

Chapter 74, "Assisting Kill", is the clearest early answer because the violence around Alec becomes final instead of hypothetical. The chapter gives readers a real answer by opening on Alec finally summoned the rest of his golems making shock spread...

Chapter 780, "Titan's Emotionless Killing Squad.", is the clearest early answer because the violence around Titan becomes final instead of hypothetical. The chapter gives readers a real answer by opening on “Move out,” Titan commanded, his voice sounding like the grinding of...